Home | ACE2 by James & Jeff

THE PROJECT

The creation of this website was assigned as the final project for the Cell and Molecular Biology lab course taught by Dr. Paramjeet S. Bagga at Ramapo College of New Jersey.

Below is the task we were given to complete:

You have purified a protein by a combination of Molecular Sieve

(Gel-Filtration), Ion-Exchange and Affinity chromatography from cultured

HeLa cells. You have been able to determine a partial amino acid sequence of

the purified protein. Your goal is to determine if the gene for the purified

protein has already been cloned, by identifying it in an appropriate protein

database and other molecular databases. If cloned, and if known, you want to

study the structure and function of the gene and the protein.

Given below is the partial amino acid sequence of the protein that was purified:

IEEQAKTFLDKFNHEAEDLFYQSSLASWNYNTNITEENVQNMNNAGDKWSAFLKEQSTLA

QMYPLQEIQNLTVKLQLQALQQNGSSVLSEDKSKRLNTILNTMSTIYSTGKVCNPDNPQE

CLLLEPGLNEIMANSLDYNERLWAWESWRSEVGKQLRPLYEEYVVLKNEMARANHYEDYG

DYWRGDYEVNGVDGYDYSRGQLIEDVEHTFEEIKPLYEHLHAYVRAKLMNAYPSYISPIG

CLPAHLLGDMWGRFWTNLYSLTVPFGQKPNIDVTDAMVDQAWDAQRIFKEAEKFFVSVGL

PNMTQGFWENSMLTDPGNVQKAVCHPTAWDLGKGDFRILMCTKVTMDDFLTAHHEMGHIQ

YDMAYAAQPFLLRNGANEGFHEAVGEIMSLSAATPKHLKSIGLLSPDFQEDNETEINFLL

KQALTIVGTLPFTYMLEKWRWMVFKGEIPKDQWMKKWWEMKREIVGVVEPVPHDETYCDP

ASLFHVSNDYSFIRYYTRTLYQFQFQEALCQAAKHEGPLHKCDISNSTEAGQKLFNMLRL

GKSEPWTLALENVVGAKNMNVRPLLNYFEPLFTWLKDQNKNSFVGWSTDWSPYADQSIKV

RISLKSALGDKAYEWNDNEMYLFRSSVAYAMRQYFLKVKNQMILFGEEDVRVANLKPRIS

FNFFVTAPKNVSDIIPRTEVEKAIRMSRSRINDAFRLNDNSLEFLGIQPTLGPPNQPPVS

IWLIVFGVVMGVIVVGIVILIFTGIRDRKKKNKARSGENPYASIDIS

OBJECTIVES

Using the above given partial amino acid sequence, the objectives for this project were…

To use bioinformatics tools and databases to identify the protein and gene of interest
To analyze and organize gene components
To identify and analyze mRNA variants and protein isoforms
To analyze and organize protein structure and function information
To assess the expression and interactions of the gene and protein
To research and report on specific mutations/diseases related to the gene and protein